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KMID : 0370219970410040414
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Yakhak Hoeji
1997 Volume.41 No. 4 p.414 ~ p.420
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Determination of Polyamines in Serum Using Benzoyl Chloride as Derivatization Agent by HPLC
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이석봉/Lee SB
고용석/김형민/문영희/진원택/신태용/Ko YS/Kim HM/Moon YH/Jin WT/Shin TY
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Abstract
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A rapid and sensitive high performance liquid chromatographic method with UV detection for the analysis of polyamines(putrescine, spermidine, spermine) has been developed. The benzoyl chloride derivatives of putrescine, spermidine and spermine are separated on a mc-Bondapak C18 reverse-phase column with 50% methanol as the mobile phase. This method was applied to the analysis of polyamines in the serum of normal human and uterine cancer patients. The results show that the mean level of polyamines in cancer patients serum is much higher than that in normal human serum.
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KEYWORD
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